To evaluate potential of the p16 INK4a 5' CpG island hypermethylation usage as an indicator of tumor cell along with p16ink4aexpression, we analyzed the methylation status of the p16 INK4a promoter region by MSP and bisulfite sequencing and expression by RT-PCR and immunohistochemical technique in HR-HPV-positive cervical tumors and cervical carcinoma cell lines. The gene discussed is CDKN2A; the disease is uterine cervix neoplasm.