PRKCE and infection: Specifically, we quantified the percentage of Lm vacuoles that recruited PKCε-YFP after infection with wild-type Lm or Lm mutants deficient in LLO (hly-), PI-PLC (plcA-), PC-PLC (plcB-) or combinations thereof (hly plcA-plcB-, plcA-plcB-).