At 5 min after infection, similar percentages were observed for wild-type (37%), hly (42%), plcA- (45%), plcB- (47%), plcA-plcB- (47%), and hly plcA-plcB- (42%) Lm phagosomes, indicating that LLO and PLCs were not necessary for PKCε recruitment during bacterial entry (Fig. 3A). This evidence concerns the gene PRKCE and infection.