MGMT and malignant glioma: In CGH analysis, the primary tumor- and the first recurrence-derived cell lines BTL1 and BTL2 exhibited several alterations characteristic of highly malignant gliomas, including loss of chromosome 10q, with the PTEN and the MGMT gene locus on 10q23.3 and 10q26, respectively, loss of material from chromosome 9p, with the CDKN2A locus on 9p16, and gain of chromosome 7 resulting in amplifications at the epidermal growth factor receptor (EGFR) locus on 7p12 (Inda et al, 2003).