The aim of this study was to determine the predictive value of EGFR in rectal cancer treated with a novel preoperative radiotherapy protocol, namely high-dose rate endorectal brachytherapy (HDREB) and its prognostic value in 1197 mismatch-repair (MMR)-proficient CRCs using the tissue microarray (TMA) technique. This evidence concerns the gene EGFR and rectal cancer.