Our results showing high level IFN-γ production from BILs derived from mice treated with vaccination + poly-ICLC administration suggest the possibility that i.m. delivery of poly-ICLC directly stimulates the functions of APCs and other immune cells infiltrating the CNS tumors, such as microglia, thereby providing the secondary stimulation of vaccine-induced Ag-specific CTLs within the CNS-tumor environment. The gene discussed is IFNG; the disease is central nervous system neoplasm.