Differentiation of monocytes utilizing membrane stimulation, as in passage through the column matrix, is advantageous in particular in the setting of cancer immunotherapy since it has been demonstrated that physical perturbation is one of the more effective means of cross-priming phagocytosed peptides into the MHC class I pathway, which is crucial in generation of a CD8 anti-tumor response [18]. This evidence concerns the gene CD8A and cancer.