In COX-2 expressing HT29 colon cancer cell lines, COX-2 inhibition with another selective COX-2 inhibitor SC236 (a structural analogue of Celecoxib) showed that at high levels of inhibitor (concentrations greater than 75 μm), COX-2 enzyme activity was completely inactivated. The gene discussed is PTGS2; the disease is malignant colon neoplasm.