To quantitate the levels of the fra-1 gene expression and validate the immunohistochemical data, we also analyzed 26 samples (2 normal breast tissues, 8 hyperplasias, 6 fibroadenomas, and 10 ductal carcinomas) for the specific fra-1 mRNA levels by a semiquantitative RT-PCR assay. The gene discussed is FOSL1; the disease is breast ductal adenocarcinoma.