As shown in Figures 4 and 5, NPM was more highly expressed in HCC (group 1), as determined by immunohistochemistry, was mainly located in the nuclei of the cancer cells, and was higher than that in the hepatocytes with non-malignant diseases (groups 2–4) in staining intensity or the total positive cell number. The gene discussed is NPM1; the disease is hepatocellular carcinoma.