In this study, we examined NPM expression in 103 paired cases of hepatocellular carcinoma (HCC), 12 cases of hepatic focal nodular hyperplasia, 17 cases of liver tissue adjacent to a hepatic haemangioma, and series of array tissues from normal human organs and malignancies using a monoclonal antibody against NPM and reverse transcription–PCR techniques, Western blot analysis, immunohistochemistry, and immunocytofluorescence. This evidence concerns the gene NPM1 and hepatocellular carcinoma.