In cholangiocarcinoma a similar pattern of expression was detected The strongest staining for both C4BP (figure 7 panel c) and CD40 was observed in the peri-tumour stroma at the margins of hepatic metastases from colorectal carcinoma where tumour cells, stromal cells, the surrounding inflammatory infiltrate and necrotic centres stained strongly for C4BP. This evidence concerns the gene CD40 and cholangiocarcinoma.