The Twist immunoreactivity categorised into negative vs positive (moderate and strong comined) expression was not associated with any of the clinicopathological parameters tested: age, FIGO stage, histological type, tumour grade, preoperative value of CA125, the intraoperative volume of ascites, residual tumour after primary cytoreductive surgery or peritoneal cytology (Table 1). This evidence concerns the gene TWIST1 and neoplasm.