AKT1 and neoplasm: Western blotting (Figure 4) was used to evaluate changes in expression of β-tubulin, HER2, p-ERK, p-AKT, p27, BCL2, AR, HSP90 and HSP70 induced by treatment with docetaxel alone or the trastuzumab plus docetaxel combination in HID28 tumours, a very high responder.