Analysis of CD4+CD25−/CD4+CD25+high T cell co-culture at a ratio of 1∶10 indicated that CD4+CD25+high T cells in control subjects showed average suppression of 46.0.±5.9% while high at-risk and recent-onset T1D subjects showed similar suppression of 10.±9.9% and 9.9±5.5% respectively, Figure 5. This evidence concerns the gene CD4 and type 1 diabetes mellitus.