Figure 3a and 3b present CD4+CD25+high T-cell apoptosis isolated from the same subjects measured by two different apoptosis assays. The apoptosis results were similar. Furthermore, correlation of apoptosis results measured by these two methods was significant Spearman's rank correlation coefficient r = 0.59 95% CI = 0.28–0.79, p = 0.0006. A higher percentage of CD4+CD25+high T cells in recent-onset T1D subjects produced more active caspase 3 than the same T-cell subtype in control subjects, confirming the ongoing apoptosis detected by YOPRO1/7AAD. This evidence concerns the gene CD4 and type 1 diabetes mellitus.