To investigate the mechanism and importance of cyclin D1 proteolysis, we first assessed the expression profile of cyclin D1 during cell cycle progression from quiescence in three normal cell lines (NIH 3T3 & WI-38 fibroblasts, and CCD841 CoN colon epithelium cells) and in three cancer cell lines (HCT 116 and SW480 colon cancers and T98G glioblastomas). The gene discussed is CCND1; the disease is malignant colon neoplasm.