To determine whether low- and high-dose 5-aza induced genes silenced by promoter methylation in NSCLC cell lines, we performed QPCR for p16. We also ran standard reverse transcriptase-PCR for p16 in several cell lines to ensure that the QPCR primer set did not amplify the alternate splice-form, p14, which is expressed in some of these cell lines [37]. Here, CDKN2A is linked to non-small cell lung carcinoma.