TP53 and Familial prostate cancer: In our study, CCNA1 was methylated in A549, which has wild-type p53, but was not methylated in NSCLC cells with mutant p53. Loss of dual-specificity phosphatase I (DUSP1) expression as determined by immunohistochemistry inversely correlates with increasing malignancy of prostate cancers, and methylation of its promoter appears to be an early event in this disease [50].