In addition, they analyzed 11 patient-matched cases with different grades of lesions with the method of LCM (laser capture microdissection) and Western blot, and demonstrated that downregulation of annexin I was an "early" event in the progression of ESCC, occurring either at the junction between high-grade DYS and invasive phenotypes or at the low-grade to high-grade DYS transition [37]. The gene discussed is ANXA1; the disease is esophageal squamous cell carcinoma.