Using at least two independent assays for the determination of EGFRvIII status, we identified the EGFRvIII allele in 28.3% (13/46) of gliomas without EGFR missense mutation and 6.3% (1/16) tumors with EGFR missense mutation (Figure 1C; Table 1); note that this tumor showed vastly lower levels of EGFRvIII (Figure 1C, lane 12). Here, EGFR is linked to glioma.