The protein expression levels and phosphorylation status of EGFR, Her2, Her3, Akt, p44/42, p38 MAP kinase, and PTEN were analyzed in all 23 lung cancer cell lines by measuring the signal intensity using NIH Image (ImageJ1.32j) (Figs. 2, 3, 4 and 5). This evidence concerns the gene PTEN and lung cancer.