Across the entire cohort of 23 lung cancer cell lines, those showing sensitivity to gefitinib exhibited greater phosphorylation of Akt and EGFR without ligand stimulation than gefitinib-resistant cell lines, according to the Mann-Whitney test (P = 0.0016, P = 0.0274, respectively) (Figs. 3 and 5). This evidence concerns the gene AKT1 and lung cancer.