Using the multiplex method for cytokine analysis of supernatants taken after 96 hours of T-cell culture, we were not able to detect peptide-specific cytokine production (all mean values of IL-1α, IL-1β, IL-5, IL-10, IL-12, IL-18, TNF-α, or IFN-γ less than 2.0 pg/ml in patients with JIA and healthy controls). The gene discussed is IL1A; the disease is juvenile idiopathic arthritis.