Previous studies suggested that the sensitivity of MSI analysis to predict an MSH6 mutation is low and that MSI should not be used as a selection criterion for MSH6 mutation analysis (Wu et al, 1999), finding microsatellite stable or low patterns in 17% up to 50% (Berends et al, 2002; Hendriks et al, 2004; Plaschke et al, 2004; Niessen et al, 2006; Pinto et al, 2006) of HNPCC-associated tumours of MSH6 mutation carriers. This evidence concerns the gene MSH6 and neoplasm.