API5 and squamous cell carcinoma: shRNA constructs to Api5 were designed, tested for their ability to deplete transfected FLAG-tagged Api5 (Figure 8A), and then expressed from lentiviral vectors (LLP) to target the endogenous Api5 protein (51-kDa doublet) in human squamous cell carcinoma 029 cells (JHU-029) (Figure 8B).