To improve sensitivity and specificity of the procedure, we and others have previously proposed a multi-marker RT-PCR assay, including melanA/MART1 in addition to tyrosinase (the use of TYR mRNA as unique marker could be of limited value in the management of MM patients, due to the heterogeneity of TYR expression in melanomas) [14-18]. The gene discussed is TYR; the disease is melanoma.