Figure 3 shows representative examples: tumours from patients #1 and #2 show strong downregulation of SASH1, whereas tumour #3 has an equal expression of SASH1 in normal and cancer tissue. Interestingly, a prominent second band with higher electrophoretic mobility (about 110 kDa) was detected in tumour lysates with the anti-SASH1 anti-serum, especially strong in normal tissue from patient #2 (Figure 3, arrow). This evidence concerns the gene SASH1 and neoplasm.