Tumour-infiltrating lymphocytes that showed maximal responses to vitronectin in the chemotaxis assay were analysed by flow cytometry, and despite gating on both CD56+ and CD3+ populations, we were unable to demonstrate αvβ3 expression on TIL (Figure 5A and B), despite strong staining of cultured dermal microvascular endothelial cells (HMEC-1; Figure 6C). This evidence concerns the gene VTN and neoplasm.