We developed both an orthotopic RCC tumor model using 104 SN12C-VHL-KD or SN12C-VC cells expressing GFP directly injected into the subcapsular region of the left kidney and a heterotopic model using 106 SN12C-VHL-KD or SN12C-VC cells expressing GFP injected into the flank of SCID mice. The gene discussed is VHL; the disease is renal cell carcinoma.