Having established the specificity of the anti-CXCL12 antibody [7,38], we next injected SN12C-VC or SN12C-VHL-KD cells directly into the subcapsular region of the left kidney of SCID mice, then treated the mice with intraperitoneal injections (500 μl) of either neutralizing goat anti-CXCL12 or preimmune serum, three times per week for 4 weeks, starting at the time of tumor cell inoculation. Here, VHL is linked to neoplasm.