In this study we analyzed: 1) LOH and MSI (microsatellite instability) at chromosome 3p21 in neuroblastic tumors; 2) promoter methylation of the RASSF1A, NORE1A, BLU and CASP8 genes, determined in neuroblastic tumors and neuroblastoma cell lines; and 3) gene expression and its correlation with promoter methylation upstream of these genes, analyzed in neuroblastoma cell lines. This evidence concerns the gene RASSF1 and neuroblastoma.