IL1B and Alzheimer disease: To further demonstrate expression and regulation of sPLA2-IIA in astrocytes, human astrocytes cultured from superior frontal gyrus of post-mortem AD brains were treated with Aβ1–42 (2.5 μM), IL-1β (20 ng/ml), and IFNγ (100 ng/ml), alone or in combination for 24 hours.