Other prominent subgroup-discriminating genes identified by RMA/RF were ABL1 for the BCR-ABL subgroup, and several B cell-specific genes with very low expression levels in T-ALL samples, including the transcription factor EBF, PAX5, a potential downstream target of EBF [34], and the transcription factor TFEB. Furthermore WNT16, a downstream target of the E2A-Pbx1 fusion protein [35], was found to be the second most important discriminator for cases with E2A-PBX1 rearrangements. This evidence concerns the gene PAX5 and acute lymphoblastic leukemia.