Figure 6A shows that Treg depletion enhances the initially weak anti-tumour responses after both ablative techniques. This effect is comparable to the effects seen with anti-CTLA-4 treatment (see also Figure 5). Treg depletion alone had no significant effect on primary tumours or re-challenges (Figure 6B). Tetramer analysis confirmed the presence of IFN-γ producing, tumour-specific T cells after the combination treatment, whereas after each individual treatment these cells were absent (Figure 6C and D). The gene discussed is CTLA4; the disease is neoplasm.