Among those 34 loci, (1) aberrant methylation of APC and p16 was detected across the three cancers; (2) MINT31 and PTEN were aberrantly methylated in both cervical cancer and ovarian cancer; (3) specifically, aberrant methylation for CDH1, DAPK, MGMT and MINT2 was only present in cervical cancer; CASP8, CDH13, hMLH1 and p73 were just methylated in endometrial cancer; aberrant methylation for BRCA1, p14, p15, RIZ and TMS1 was only detected in ovarian cancer. This evidence concerns the gene MLH1 and cancer.