The sensitivity of the method did not enable us to identify a differential immuno-staining for molecules whose RT-PCR expression showed differences ranging from 42% (CTGF up-regulation in SSc-MVECs, Table 1) to 185% (IL8 up-regulation in SSc-MVECs, Table 1), while all the tissue KLKs and DSG2, whose down-regulation in SSc-MVECs ranged from 10.63-fold to 53.07-fold, exhibited a measurable differential staining (Figure 1). This evidence concerns the gene CCN2 and systemic sclerosis.