To test whether hypoxia-induced expression of NDRG1 is dependent on differentiation, two pancreatic cancer cells lines, the moderately differentiated Capan-1 and the poorly differentiated Panc-1, were cultured either at 21% O2 (normoxia, N) or at 2% O2 (hypoxia, H). The gene discussed is NDRG1; the disease is pancreatic neoplasm.