Clone PR3 secreted interferon-γ in response to WMPG, the MAGE-3-transfected HLA-DR11+ EBV-transformed cell line and three of four HLA-DR11+ melanoma cell lines, but did not secrete interferon-γ after stimulation with either of the HLA-DR11− melanoma lines or after stimulation with an HLA-DR11+ line (LB33) with low HLA-DR expression (Figure 5B and data not shown). The gene discussed is PRTN3; the disease is melanoma.