It is therefore of great interest that unsupervised clustering of the miRNA profiles from the 20 breast cancer samples studied here clearly discriminated at least two subsets of ErbB2-positive breast cancers, one that is largely ErbB2-positive and ER-negative, and another more remotely related group of ErbB2-positive cancers with miRNA features more typical of ER-positive breast cancers. The gene discussed is ESR1; the disease is breast carcinoma.