Before vaccination mice received in vitro stimulated (IVS) pmel transgenic (Tg) CD8+ T cells, which have a T-cell receptor specific for the murine H-2Db restricted gp10025–33 peptide of the B16F10 melanoma associated gp100 protein, and strongly cross react with hgp10025–33 [10]. This evidence concerns the gene CD8A and melanoma.