Most of these experiments have focused on the adoptive transfer of antigen-educated pmel CD8+ splenocytes stimulated in vitro with hgp10025–33 peptide and IL-2, and the subsequent in vivo expansion of these highly activated cells in tumor-bearing mice with hgp10025–33 vaccination and follow-on cytokine therapy [3,16-18]. This evidence concerns the gene CD8A and neoplasm.