This was performed on three groups of sheep: firstly, sheep from an early and late scrapie pathogenesis study with mainly genotype VRQ/VRQ (only codons 136, 154 and 171 of PrP are indicated in single letter amino acid code); secondly, ARQ/ARQ sheep with orally induced BSE; and thirdly, sheep diagnosed during active and passive surveillance in the years 2002–2003. This evidence concerns the gene PRNP and scrapie.