GJA1 and infection: In an attempt to minimise the adverse effect of the transduction inhibitors, we used various dilutions of the MLV-CX43-EGFP viral vector preparation with end-point titre 2 × 105 TU/ml for infection of the primary rat skeletal myoblasts in the presence of 5 μg/ml of the commonly used transduction enhancer polycation PolybreneTM (hexadimethrine bromide).