Quantitative RT–PCR analysis revealed the transcription of AT1 and AT2 receptor mRNA in both normal and diseased human breast tissues though AT1 mRNA was much more abundant in carcinoma than in normal tissue (Figure 1A and B), thus supporting the growing body of evidence discussed previously that increased AT1 receptor expression may contribute to mammary carcinoma (Greco et al, 2002a). This evidence concerns the gene AGTR1 and carcinoma.