In order to measure the levels of viral cDNA synthesis in the presence of overexpressed Rad18, Hela cells transfected with Rad18-IRES HcRed-expressing plasmid or HcRed control were infected with VSV-G pseudotyped integration-deficient HIV-1 retrovirus and sorted at 12 and 20 h post-infection. The gene discussed is RAD18; the disease is infection.