In contrast, stimulation with 5% FBS, a commonly used HASM cell stimulus [24,31], increased the production of IL-8 in both asthmatic and non-asthmatic derived HASM cells in comparison to constitutive release, and infection with RV-16 (MOI 4) and concurrent stimulation with 5% FBS significantly induced the release of IL-8 in comparison to 5% FBS alone in HASM cells derived from both asthmatic and non-asthmatic subjects. The gene discussed is CXCL8; the disease is infection.