However, when ERα-positive cases were considered separately (n=225), there was a strong negative correlation between the presence of AGR2 staining (using a 1% cutoff of the carcinoma cells stained) and patient survival, with patients whose tumours were AGR2 positive having significantly poorer survival than those whose tumours were AGR2 negative (Figure 3B; log rank test, P=0.007; Wilcoxon test, χ2=6.0, 1d.f., P=0.01). Here, ESR1 is linked to carcinoma.