We performed chromatin immunoprecipitation (ChIP) assays in MDA-MB-231 cells and observed SIRT1 localization at DNA-hypermethylated and silenced promoters for SFRP1, E-cadherin, and GATA-5 (Figure 5 and unpublished data) and at the silenced MLH1 and E-cadherin promoters in RKO colon cancer cells (Figure 5C). Here, SFRP1 is linked to malignant colon neoplasm.