Western blot analysis of the control SW480 cell line, non-neoplastic temporal lobe tissue and nine brain tumour total protein extracts (including five strong expressers of ADAMTS-8 by IHC analysis, see Table 1) using the same ADAMTS-8 primary antibody revealed one specific band at ∼98 kDa in the SW480 control, temporal lobe and three glioma extracts (all IHC positive) (Figure 3, Table 1). Here, ADAMTS8 is linked to brain neoplasm.