LGALS4 and neoplasm: Following validation of the transcript profiling results using RT–PCR analysis on ovarian cancer extracts of varying histological subtypes, we determined the protein expression of one such candidate tumour marker, galectin 4 (LGALS4), in primary ovarian tissue (normal surface epithelium, benign mucinous cysts, mucinous borderline tumours and ovarian carcinomas) using high-throughput immunohistochemistry based on tissue microarrays.