The main IGFBPs were determined by Western-ligand-blot, showing changes in the pattern of IGFBPs with a significant increment of IGFBP3 in rats with testicular atrophy treated with IGF-I (Arbitrary Units, CO: 23.77 ± 0.48, AT: 23.01 ± 0.95, AT+IGF: 28.24 ± 1.11) (see Figure 5). The gene discussed is IGFBP3; the disease is Testicular atrophy.