The same mechanisms appeared also to be operating in ER-positive MCF-7 breast cancer cells: paclitaxel at the same concentration caused JNK activation/FADD phosphorylation, and cell cycle arrest at G2/M, which were cancelled by the JNK inhibitor or S194A mutant FADD overexpression (Figure 3A, right panels) (Figure 3B, lower panels) (Figure 3C, right bars) (Figure 3D black bars). This evidence concerns the gene MAPK8 and breast cancer.