BRCA1 and breast carcinoma: Using rigorously controlled semiquantitative RT–PCR and densitometry analysis, we found that a dose of I3C (60 μM) that causes little or no cytotoxicity caused a time-dependent increase in BRCA1 and BRCA2 mRNA levels in two breast cancer cell lines, MCF-7 and T47D (Figure 1A and B).