In this study, we compared the anti-proliferative and apoptotic effects of RRR-α-tocopherol and RRR-γ-tocopherol in four colon cancer cell lines with varying molecular characteristics, SW480 (APC, type I truncation and COX-2 deficient), HCT-15 (COX-2 deficient), HCT-116 (APC, wild-type and COX-2 inducible), and HT-29 (APC, type II truncation and COX-2 constitutive expression) and normal untransformed colon cells (CCD-12CoN). This evidence concerns the gene APC and malignant colon neoplasm.