We further tested whether in vivo stimulation with a TLR agonist, PolyI:C or R837, could further enhance anti-gp120 immune responses, since SOCS1 is an inducible feedback inhibitor [15,16] and immune responses against tumor-associated antigens induced by SOCS1-silenced DCs were preferentially enhanced by in vivo stimulation with LPS in our previous study [24]. Here, SOCS1 is linked to neoplasm.