The analysis of the neuropathological lesion pattern was performed according to published protocols [8,13] with the purpose to: (i) evaluate whether immunohistochemical PrP staining and histological lesions (gliosis, spongiosis) correlate with the absolute amount of biochemically assessed PrPSc, and (ii) compare whether lesion profiles generated by histological assessment parallel biochemically defined PrPSc profiles. The gene discussed is PRNP; the disease is Gliosis.